pGGB-SpCas9
(Plasmid
#210533)
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PurposeCloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210533 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJet 1.3
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Backbone manufacturerThermo Scientific
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpCas9
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SpeciesOther
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGGB-SpCas9 was a gift from Ortrun Mittelsten Scheid (Addgene plasmid # 210533 ; http://n2t.net/addgene:210533 ; RRID:Addgene_210533) -
For your References section:
A versatile CRISPR-based system for lineage tracing in living plants. Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M, Dolan L, Mittelsten Scheid O. Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378. 10.1111/tpj.16378 PubMed 37403571