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pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR
(Plasmid #210523)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210523 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    GFP-KASH
  • Insert Size (bp)
    982

Gene/Insert 2

  • Gene/Insert name
    U6-gRNA-directcapture
  • Insert Size (bp)
    365

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.18.558077 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR was a gift from Xin Jin (Addgene plasmid # 210523 ; http://n2t.net/addgene:210523 ; RRID:Addgene_210523)

  • For your References section:

    Massively parallel in vivo Perturb-seq reveals cell-type-specific transcriptional networks in cortical development. Zheng X, Wu B, Liu Y, Simmons SK, Kim K, Clarke GS, Ashiq A, Park J, Li J, Wang Z, Tong L, Wang Q, Rajamani KT, Munoz-Castaneda R, Mu S, Qi T, Zhang Y, Ngiam ZC, Ohte N, Hanashima C, Wu Z, Xu X, Levin JZ, Jin X. Cell. 2024 Jun 20;187(13):3236-3248.e21. doi: 10.1016/j.cell.2024.04.050. Epub 2024 May 20. 10.1016/j.cell.2024.04.050 PubMed 38772369
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