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Addgene

pDestTol2pA ins:Apollo-NADP+ mVenus
(Plasmid #210518)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210518 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDestTol2pA
  • Total vector size (bp) 8127
  • Vector type
    Zebrafish expression, Tol2 kit Gateway-compatible

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Apollo-NADP+
  • Species
    Synthetic
  • Insert Size (bp)
    2277
  • Promoter Zebrafish insulin promoter
  • Tag / Fusion Protein
    • mVenus (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Zebrafish insulin promoter cloned from ins:CFP-NTR (Addgene plasmid # 66593 ; http://n2t.net/addgene:66593 ; RRID:Addgene_66593)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDestTol2pA ins:Apollo-NADP+ mVenus was a gift from Jonathan Rocheleau (Addgene plasmid # 210518 ; http://n2t.net/addgene:210518 ; RRID:Addgene_210518)

  • For your References section:

    Apollo-NADP(+) reveals in vivo adaptation of NADPH/NADP(+) metabolism in electrically activated pancreatic beta cells. Bui CV, Boswell CW, Ciruna B, Rocheleau JV. Sci Adv. 2023 Oct 6;9(40):eadi8317. doi: 10.1126/sciadv.adi8317. Epub 2023 Oct 4. 10.1126/sciadv.adi8317 PubMed 37792934
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