pAAV-cFOS-FRB-VP16-P2A-EGFP
(Plasmid #210510)

• Purpose: expresses cFOS-FRB-VP16 and EGFP component in rat hippocampal neuron cells
• Depositing Lab: Dongmin Lee
• Publication: Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. doi: 10.1038/s41589-023-01480-6. Epub 2023 Nov 16.

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Backbone

• Vector backbone: pUC
• Total vector size (bp): 7372
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FRB-VP16-P2A-EGFP
• Insert Size (bp): 1551
• Promoter: cFos

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: ggatcggccgccacc
• 3′ sequencing primer: atggatggataattc

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-1628455/v1 for preprint.