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hSyn-PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP
(Plasmid #210509)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210509 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSyc181 P2A
  • Total vector size (bp) 6900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    hSyn promoter
  • Entrez Gene
    RIC8B (a.k.a. RIC8, hSyn)

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer attacgccagtcgactAGTGCAAGTGGGTTTTAG
  • 3′ sequencing primer aagcttCTGCGCTCTCAGGCAC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP
  • Insert Size (bp)
    3405
  • Promoter hSyn
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer AAA TGG GCG GTA GGC GTG
  • 3′ sequencing primer GAA ACA GCT ATG ACC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-1628455/v1 for preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hSyn-PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP was a gift from Dongmin Lee (Addgene plasmid # 210509 ; http://n2t.net/addgene:210509 ; RRID:Addgene_210509)

  • For your References section:

    A single-component, light-assisted uncaging switch for endoproteolytic release. Cui M, Lee S, Ban SH, Ryu JR, Shen M, Yang SH, Kim JY, Choi SK, Han J, Kim Y, Han K, Lee D, Sun W, Kwon HB, Lee D. Nat Chem Biol. 2024 Mar;20(3):353-364. doi: 10.1038/s41589-023-01480-6. Epub 2023 Nov 16. 10.1038/s41589-023-01480-6 PubMed 37973890
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