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IZ1181
(Plasmid #21049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21049 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1Zeo(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a or equivallent
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human MDM2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1494
  • Mutation
    no mutation. This is the wild-type human MDM2.
  • GenBank ID
    M92424
  • Entrez Gene
    MDM2 (a.k.a. ACTFS, HDMX, LSKB, hdm2)
  • Tag / Fusion Protein
    • no tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Source: HeLa quick-clone cDNA (Clontech)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IZ1181 was a gift from Tadahide Izumi (Addgene plasmid # 21049 ; http://n2t.net/addgene:21049 ; RRID:Addgene_21049)

  • For your References section:

    Ubiquitination of mammalian AP endonuclease (APE1) regulated by the p53-MDM2 signaling pathway. Busso CS, Iwakuma T, Izumi T. Oncogene. 2009 Apr 2. 28(13):1616-25. 10.1038/onc.2009.5 PubMed 19219073
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