pKB12 (pDEST-DHFR F[3] C-term, HygR)
(Plasmid
#210486)
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Purpose(Empty Backbone) pDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 210486 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDN0510
- Backbone size (bp) 9610
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Modifications to backboneReplaced Yeast selection marker from LEU2 to Hygromycin B resistance cassette.
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Vector typeYeast Expression, Synthetic Biology ; Destination vector for Gateway cloning
- Promoter ADH1
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsThe chloramphenicol resistance gene in the Gateway cassette is broken.
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKB12 (pDEST-DHFR F[3] C-term, HygR) was a gift from Christian Landry (Addgene plasmid # 210486 ; http://n2t.net/addgene:210486 ; RRID:Addgene_210486) -
For your References section:
Parallel nonfunctionalization of CK1delta/epsilon kinase ohnologs following a whole-genome duplication event. Evans-Yamamoto D, Dube AK, Saha G, Plante S, Bradley D, Gagnon-Arsenault I, Landry CR. Mol Biol Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246. 10.1093/molbev/msad246 PubMed 37979156
Map uploaded by the depositor.
