pDN0617 (pDEST-mEGFP C-term, NatR)
(Plasmid #210484)

• Purpose: (Empty Backbone) pDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.
• Depositing Lab: Christian Landry
• Publication: Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246.

Backbone

• Vector backbone: pDN0603
• Backbone size (bp): 11125
• Modifications to backbone: Inserted mEGFP for C-terminal fusion to gene of interest.
• Vector type: Yeast Expression, Synthetic Biology ; Destination vector for Gateway cloning
• Promoter: ADH1
• Selectable markers: Nourseothricin
• Tag / Fusion Protein:
  • mEGFP (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • pdn0617.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.