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Addgene

pDN0520 (pDEST, TRP1)
(Plasmid #210481)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210481 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDN0509
  • Backbone size (bp) 10080
  • Modifications to backbone
    Removed the DHFR F[1,2] which was flanking the C-terminus of the Gateway cassette.
  • Vector type
    Destination vector for Gateway cloning
  • Promoter ADH1
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDN0520 (pDEST, TRP1) was a gift from Christian Landry (Addgene plasmid # 210481 ; http://n2t.net/addgene:210481 ; RRID:Addgene_210481)

  • For your References section:

    Parallel nonfunctionalization of CK1delta/epsilon kinase ohnologs following a whole-genome duplication event. Evans-Yamamoto D, Dube AK, Saha G, Plante S, Bradley D, Gagnon-Arsenault I, Landry CR. Mol Biol Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246. 10.1093/molbev/msad246 PubMed 37979156
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