pEGFP_PCNA_IRES_puro2b
(Plasmid #21048)

• Depositing Lab: Daniel Gerlich
• Publication: Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Backbone

• Vector backbone: IRES_puro2b
• Backbone size w/o insert (bp): 6037
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hPCNA
• Species: H. sapiens (human)
• Insert Size (bp): 758
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: -

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry