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Addgene

Cas9-T2A-H2B mCitirine
(Plasmid #210471)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210471 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC
  • Backbone manufacturer
    VectorBuilder
  • Backbone size w/o insert (bp) 2390
  • Total vector size (bp) 7867
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-T2A-H2B mCitirine
  • Species
    H. sapiens (human), Synthetic; Aequorea victoria, streptococcus pyogenes
  • Insert Size (bp)
    7804
  • Mutation
    Cas9 contains 3xFLAG tag and nuclear localization signal
  • Tag / Fusion Protein
    • mCitrine on C terminal of H2B

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ATAAGGGCGACACGGAAATG
  • 3′ sequencing primer CTCGCTCACTGACTCGCT
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    VectorBuilder

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cas9-T2A-H2B mCitirine was a gift from Amro Hamdoun (Addgene plasmid # 210471 ; http://n2t.net/addgene:210471 ; RRID:Addgene_210471)
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