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Addgene

pmPAGFP_IRES_puro2
(Plasmid #21046)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21046 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    IRES_puro2b
  • Backbone size w/o insert (bp) 5079
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pmPAGFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    722

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site - (unknown if destroyed)
  • 3′ cloning site - (unknown if destroyed)
  • 5′ sequencing primer -
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmPAGFP_IRES_puro2 was a gift from Daniel Gerlich (Addgene plasmid # 21046 ; http://n2t.net/addgene:21046 ; RRID:Addgene_21046)

  • For your References section:

    Aurora B-mediated abscission checkpoint protects against tetraploidization. Steigemann P, Wurzenberger C, Schmitz MH, Held M, Guizetti J, Maar S, Gerlich DW. Cell. 2009 Feb 6. 136(3):473-84. 10.1016/j.cell.2008.12.020 PubMed 19203582
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