pWaaTW-R4
(Plasmid
#210456)
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PurposeExpress waaT and waaW from E. coli R4 under the control of PBAD
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 210456 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBAD30
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Backbone manufacturerGuzman et al. 1995 PMID: 7608087
- Backbone size w/o insert (bp) 4919
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namewaaT and waaW
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SpeciesE. coli F2513 (R4)
- Promoter PBAD
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBAD_fwd_primer
- 3′ sequencing primer pBAD_rev_primer (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWaaTW-R4 was a gift from Sébastien Rodrigue (Addgene plasmid # 210456 ; http://n2t.net/addgene:210456 ; RRID:Addgene_210456) -
For your References section:
Systematic investigation of recipient cell genetic requirements reveals important surface receptors for conjugative transfer of IncI2 plasmids. Allard N, Collette A, Paquette J, Rodrigue S, Cote JP. Commun Biol. 2023 Nov 16;6(1):1172. doi: 10.1038/s42003-023-05534-2. 10.1038/s42003-023-05534-2 PubMed 37973843
