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Addgene

pWaaW
(Plasmid #210454)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210454 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD30
  • Backbone manufacturer
    Guzman et al. 1995 PMID: 7608087
  • Backbone size w/o insert (bp) 4919
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    waaW
  • Species
    E. coli Nissle (R1-type)
  • Promoter PBAD

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer pBAD_fwd_primer
  • 3′ sequencing primer pBAD_rev_primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWaaW was a gift from Sébastien Rodrigue (Addgene plasmid # 210454 ; http://n2t.net/addgene:210454 ; RRID:Addgene_210454)

  • For your References section:

    Systematic investigation of recipient cell genetic requirements reveals important surface receptors for conjugative transfer of IncI2 plasmids. Allard N, Collette A, Paquette J, Rodrigue S, Cote JP. Commun Biol. 2023 Nov 16;6(1):1172. doi: 10.1038/s42003-023-05534-2. 10.1038/s42003-023-05534-2 PubMed 37973843
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