pETM43 p62 FL (1-440)
(Plasmid
#210425)
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PurposeBacterial expression of MBP-p62 FL (1-440) with 3C cleavage site
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210425 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepETM43
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namep62 FL
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SpeciesH. sapiens (human)
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Entrez GeneNUP62 (a.k.a. IBSN, SNDI, p62)
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Tags
/ Fusion Proteins
- MBP (N terminal on backbone)
- 6xHis (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETM43 p62 FL (1-440) was a gift from Carsten Sachse (Addgene plasmid # 210425 ; http://n2t.net/addgene:210425 ; RRID:Addgene_210425) -
For your References section:
Recombinant Expression, Purification, and Assembly of p62 Filaments. Tarafder AK, Guesdon A, Kuhm T, Sachse C. Methods Mol Biol. 2019;1880:3-15. doi: 10.1007/978-1-4939-8873-0_1. 10.1007/978-1-4939-8873-0_1 PubMed 30610687