pmRFP_a_tubulin_IRES_puro2b
(Plasmid #21041)

• Depositing Lab: Daniel Gerlich
• Publication: Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Backbone

• Vector backbone: IRES_puro2b
• Backbone size w/o insert (bp): 5776
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: alpha Tubulin
• Alt name: tubulin, alpha 1b
• Species: H. sapiens (human)
• Insert Size (bp): 1360
• Entrez Gene: TUBA1B (a.k.a. K-ALPHA-1)
• Promoter: CMV
• Tag / Fusion Protein:
  • pmRFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: IRES-R

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry