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PRS_8-GCaMP8m
(Plasmid #210400)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210400 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV2
  • Backbone size w/o insert (bp) 4093
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCaMP8m
  • Species
    Synthetic
  • Insert Size (bp)
    1269
  • Promoter PRSx8

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer aacgcgtattagcttccgctag
  • 3′ sequencing primer aagcaatagcatgatacaaagg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.01.22.477348 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PRS_8-GCaMP8m was a gift from Simon Wiegert (Addgene plasmid # 210400 ; http://n2t.net/addgene:210400 ; RRID:Addgene_210400)

  • For your References section:

    Targeting Norepinephrine Neurons of the Locus Coeruleus: A Comparison of Model Systems and Strategies. Wissing C, Eschholz LS, Maheu M, Sauter K, Morellini F, Wiegert S, Dieter A. bioRxiv 2022.01.22.477348 10.1101/2022.01.22.477348
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