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pET303_sumocodo_GFP_codoopti
(Plasmid #210393)

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Item Catalog # Description Quantity Price (USD)
Plasmid 210393 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET303
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6437
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21(DE3) strain for protein expression
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP
  • Insert Size (bp)
    1068
  • GenBank ID
    AAA27722.1
  • Tag / Fusion Protein
    • SUMO-tag (N terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET303_sumocodo_GFP_codoopti was a gift from Magnus Rueping (Addgene plasmid # 210393 ; http://n2t.net/addgene:210393 ; RRID:Addgene_210393)

  • For your References section:

    Nanobody-Based Lateral Flow Immunoassay for Rapid Antigen Detection of SARS-CoV-2 and MERS-CoV Proteins. Peng Y, Huang Y, Kiessling F, Renn D, Rueping M. ACS Synth Biol. 2025 Jan 9. doi: 10.1021/acssynbio.4c00592. 10.1021/acssynbio.4c00592 PubMed 39786915
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