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Addgene

SGTA-EGFP
(Plasmid #210373)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210373 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5639
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SGTA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    939
  • GenBank ID
    NM_003021.4
  • Entrez Gene
    SGTA (a.k.a. SGT, SGT1, Vpu, alphaSGT, hSGT)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP (Cterm)
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Horizon Discovery MHS6278-202825921

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SGTA-EGFP was a gift from Michele Pagano (Addgene plasmid # 210373 ; http://n2t.net/addgene:210373 ; RRID:Addgene_210373)

  • For your References section:

    The REEP5/TRAM1 complex binds SARS-CoV-2 NSP3 and promotes virus replication. Li J, Gui Q, Liang FX, Sall J, Zhang Q, Duan Y, Dhabaria A, Askenazi M, Ueberheide B, Stapleford KA, Pagano M. J Virol. 2023 Sep 28:e0050723. doi: 10.1128/jvi.00507-23. 10.1128/jvi.00507-23 PubMed 37768083
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