TRAM1-EGFP
(Plasmid #210365)

• Purpose: Expresses TRAM1 fused with EGFP in mammalian cells
• Depositing Lab: Michele Pagano
• Publication: Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1128/jvi.00507-23.

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5800
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRAM1
• Species: H. sapiens (human)
• Insert Size (bp): 1122
• GenBank ID:
• Entrez Gene: TRAM1 (a.k.a. PNAS8, TRAM, TRAMP)
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP (Cterm)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Horizon Discovery MHS6278-202807922

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry