Flag-V0A
(Plasmid #210347)

• Purpose: Expresses ATP6V0A in mammalian cells
• Depositing Lab: Michele Pagano
• Publication: Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1128/jvi.00507-23.

Backbone

• Vector backbone: PcDNA3.1
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 7200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: V-ATPase V0A
• Species: H. sapiens (human)
• GenBank ID:
• Entrez Gene: ATP6V0A1 (a.k.a. ATP6N1, ATP6N1A, Stv1, VPP1, Vph1, a1)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHR

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dafna Bar-Sagi

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry