pXTm-zebrafish Tspan4a
(Plasmid
#210327)
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PurposeExpresses Tetraspanin-4a (TSPAN4a) as migrasome marker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210327 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepXT7
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Vector typeFish Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTetraspanin 4a
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SpeciesD. rerio (zebrafish)
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Entrez Genetspan4a (a.k.a. zgc:92174)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXTm-zebrafish Tspan4a was a gift from Li Yu (Addgene plasmid # 210327 ; http://n2t.net/addgene:210327 ; RRID:Addgene_210327) -
For your References section:
Migrasomes provide regional cues for organ morphogenesis during zebrafish gastrulation. Jiang D, Jiang Z, Lu D, Wang X, Liang H, Zhang J, Meng Y, Li Y, Wu D, Huang Y, Chen Y, Deng H, Wu Q, Xiong J, Meng A, Yu L. Nat Cell Biol. 2019 Aug;21(8):966-977. doi: 10.1038/s41556-019-0358-6. Epub 2019 Aug 1. 10.1038/s41556-019-0358-6 PubMed 31371827