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Addgene

pEGFP-N1-Tspan4
(Plasmid #210323)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210323 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tetraspanin 4
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Tspan4 (a.k.a. Tm4sf7)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Sal1 (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1-Tspan4 was a gift from Li Yu (Addgene plasmid # 210323 ; http://n2t.net/addgene:210323 ; RRID:Addgene_210323)

  • For your References section:

    Discovery of the migrasome, an organelle mediating release of cytoplasmic contents during cell migration. Ma L, Li Y, Peng J, Wu D, Zhao X, Cui Y, Chen L, Yan X, Du Y, Yu L. Cell Res. 2015 Jan;25(1):24-38. doi: 10.1038/cr.2014.135. Epub 2014 Oct 24. 10.1038/cr.2014.135 PubMed 25342562
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