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NSP4-mCherry
(Plasmid #210319)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210319 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mCherry-N1
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 NSP4
  • Species
    H. sapiens (human); SARS-CoV-2 isolate Wuhan-Hu-1
  • Insert Size (bp)
    1506
  • Mutation
    Many synonymous changes due to codon optimization
  • GenBank ID
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer mCherry-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NSP4-mCherry was a gift from Michele Pagano (Addgene plasmid # 210319 ; http://n2t.net/addgene:210319 ; RRID:Addgene_210319)

  • For your References section:

    The REEP5/TRAM1 complex binds SARS-CoV-2 NSP3 and promotes virus replication. Li J, Gui Q, Liang FX, Sall J, Zhang Q, Duan Y, Dhabaria A, Askenazi M, Ueberheide B, Stapleford KA, Pagano M. J Virol. 2023 Sep 28:e0050723. doi: 10.1128/jvi.00507-23. 10.1128/jvi.00507-23 PubMed 37768083
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