NSP6-FR-2Strep
(Plasmid #210317)

• Purpose: Expresses SARS-CoV-2 NSP6 in mammalian cells
• Depositing Lab: Michele Pagano
• Publication: Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1128/jvi.00507-23.

Backbone

• Vector backbone: PcDNA3.1
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6200
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2 NSP6
• Species: SARS-CoV-2 isolate Wuhan-Hu-1
• Insert Size (bp): 876
• Mutation: Many synonymous changes due to codon optimization
• GenBank ID:
• Entrez Gene: ORF1ab (a.k.a. GU280_gp01)
• Tag / Fusion Protein:
  • 2Strep (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHR

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Fritz Roth, Addgene plasmid #154405

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry