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pMXS-GshF-blasticidin
(Plasmid #210313)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210313 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMXS-IRES-Blast
  • Backbone manufacturer
    Cell Biolabs
  • Backbone size w/o insert (bp) 5600
  • Total vector size (bp) 7910
  • Vector type
    Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF
  • Alt name
    GshF
  • Alt name
    GshAB
  • Species
    Streptococcus thermophilus
  • Insert Size (bp)
    3913
  • Mutation
    Codon optimized for expression in human cells
  • GenBank ID
    CP000419, AAGS01000000, AAGS01000001-AAGS01000098, NZ_LR822015.1
  • Entrez Gene
    gshAB (a.k.a. H0506_RS06410, STHERMO_1423)
  • Tag / Fusion Protein
    • FLAG peptide (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Humanized from the Streptococcus thermophilus GshF sequence initially cloned by Li et. al (PMID: 21683099)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXS-GshF-blasticidin was a gift from Kivanc Birsoy (Addgene plasmid # 210313 ; http://n2t.net/addgene:210313 ; RRID:Addgene_210313)

  • For your References section:

    SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells. Wang Y, Yen FS, Zhu XG, Timson RC, Weber R, Xing C, Liu Y, Allwein B, Luo H, Yeh HW, Heissel S, Unlu G, Gamazon ER, Kharas MG, Hite R, Birsoy K. Nature. 2021 Nov;599(7883):136-140. doi: 10.1038/s41586-021-04025-w. Epub 2021 Oct 27. 10.1038/s41586-021-04025-w PubMed 34707288
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