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Addgene

ECFP-FRB-Sec61b
(Plasmid #210290)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210290 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ECFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ECFP-FRB-Sec61b
  • Species
    Synthetic
  • Promoter CMV
  • Tags / Fusion Proteins
    • ECFP
    • FRB

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ECFP-FRB-Sec61b was a gift from Takanari Inoue (Addgene plasmid # 210290 ; http://n2t.net/addgene:210290 ; RRID:Addgene_210290)

  • For your References section:

    ActuAtor, a Listeria-inspired molecular tool for physical manipulation of intracellular organizations through de novo actin polymerization. Nakamura H, Rho E, Lee CT, Itoh K, Deng D, Watanabe S, Razavi S, Matsubayashi HT, Zhu C, Jung E, Rangamani P, Watanabe S, Inoue T. Cell Rep. 2023 Sep 2:113089. doi: 10.1016/j.celrep.2023.113089. 10.1016/j.celrep.2023.113089 PubMed 37734382
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