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Addgene

pSWEET-bgaB
(Plasmid #210272)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210272 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSWEET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli Novablues
  • Growth instructions
    Use Chloramphenicol (10 ug/ml) for growth in Bacillus
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    bgaB
  • Species
    B. stearothermophilus
  • Promoter PxylA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSWEET-bgaB was a gift from Eric Brown (Addgene plasmid # 210272 ; http://n2t.net/addgene:210272 ; RRID:Addgene_210272)

  • For your References section:

    Development and characterization of a xylose-dependent system for expression of cloned genes in Bacillus subtilis: conditional complementation of a teichoic acid mutant. Bhavsar AP, Zhao X, Brown ED. Appl Environ Microbiol. 2001 Jan;67(1):403-10. doi: 10.1128/AEM.67.1.403-410.2001. 10.1128/AEM.67.1.403-410.2001 PubMed 11133472
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