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CBP-Bromo-HAT-CH3-pET
(Plasmid #21027)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21027 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CBP
  • Alt name
    CREB-binding protein HAT domain
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2337
  • Mutation
    contains HAT domain aa 1172-1621
  • Entrez Gene
    Crebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • T7 tag (N terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    R. Goodman and T. Kouzarides

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CBP-Bromo-HAT-CH3-pET was a gift from Corey Largman (Addgene plasmid # 21027)

  • For your References section:

    The HOX homeodomain proteins block CBP histone acetyltransferase activity. Shen WF, Krishnan K, Lawrence HJ, Largman C. Mol Cell Biol. 2001 Nov;21(21):7509-22 10.1128/MCB.21.21.7509-7522.2001 PubMed 11585930
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