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pBES1-LacOx50-mCh-bla
(Plasmid #210260)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210260 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2257
  • Total vector size (bp) 7491
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    50xLacO repeats derived from: PMID: 12864855.
  • Species
    Synthetic; E. coli
  • Insert Size (bp)
    1887

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    708

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Digest with AvrII + KpnI for transfection into trypanosomes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBES1-LacOx50-mCh-bla was a gift from Calvin Tiengwe (Addgene plasmid # 210260 ; http://n2t.net/addgene:210260 ; RRID:Addgene_210260)

  • For your References section:

    Imaging of genomic loci in Trypanosoma brucei using an optimised LacO-LacI system. Budzak J, Goodwin I, Tiengwe C, Rudenko G. Mol Biochem Parasitol. 2023 Dec;256:111598. doi: 10.1016/j.molbiopara.2023.111598. Epub 2023 Nov 3. 10.1016/j.molbiopara.2023.111598 PubMed 37923299
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