CBP-Bromo-HAT-CH3-sp65
(Plasmid #21026)

• Depositing Lab: Corey Largman
• Publication: Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Backbone

• Vector backbone: pSP65
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3000
• Vector type: in vitro TNT

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CBP
• Alt name: CREB-binding protein Bromo, HAT and CH3 domains
• Species: M. musculus (mouse)
• Insert Size (bp): 2337
• Mutation: Bromo+HAT+CH3 domains aa 1098-1877; E1239G mutation
• Entrez Gene: Crebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: M13_rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: R. Goodman and T. Kouzarides

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry