MSX2-pET
(Plasmid #21025)

• Depositing Lab: Corey Largman
• Publication: Stelnicki et al Differentiation. 1997 Oct . 62(1):33-41.

Backbone

• Vector backbone: pET-28c
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5376
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MSX2
• Species: M. musculus (mouse)
• Insert Size (bp): 1000
• Entrez Gene: Msx2 (a.k.a. BB122635, Hox, Hox-, Hox-8, Hox8, Hox8., Hox8.1)
• Tags / Fusion Proteins:
  • T7 (N terminal on backbone)
  • HIS (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Yi Hsin Liu
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry