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Addgene

MSX1-pET
(Plasmid #21024)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21024 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MSX1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Entrez Gene
    Msx1 (a.k.a. Hox-7, Hox7, Hox7.1, msh)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • T7 tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSX1-pET was a gift from Corey Largman (Addgene plasmid # 21024 ; http://n2t.net/addgene:21024 ; RRID:Addgene_21024)

  • For your References section:

    The human homeobox genes MSX-1, MSX-2, and MOX-1 are differentially expressed in the dermis and epidermis in fetal and adult skin. Stelnicki EJ, Koemuves LG, Holmes D, Clavin W, Harrison MR, Adzick NS, Largman C. Differentiation. 1997 Oct . 62(1):33-41. 10.1046/j.1432-0436.1997.6210033.x PubMed 9373945
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