pSMOX-mNG::LacI-V3
(Plasmid #210224)

• Purpose: Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.
• Depositing Lab: Calvin Tiengwe
• Publication: Budzak et al Mol Biochem Parasitol. 2023 Dec;256:111598. doi: 10.1016/j.molbiopara.2023.111598. Epub 2023 Nov 3.

Backbone

• Vector backbone: pSMOX
• Backbone manufacturer: Generated in: PMID: 22645659
• Backbone size w/o insert (bp): 8422
• Total vector size (bp): 10198
• Vector type: Trypanosoma brucei expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mNeongreen Lac repressor fusion protein
• Insert Size (bp): 1710
• Promoter: Pol II readthrough transcription after integration

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The mNeongreen sequence was amplified from the pPOT plasmid series and was obtained from Sam Dean PMID: 25567099. The LacI sequence was obtained from Addgene #59481.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Digest with HindIII for transfection into trypanosomes.