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Addgene

prDNA spacer-LacOx120-20xMS2-Hyg
(Plasmid #210220)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210220 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2413
  • Total vector size (bp) 10843
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    120xLacO repeats derived from: PMID: 12864855.
  • Species
    Synthetic; E. coli
  • Insert Size (bp)
    4453

Gene/Insert 2

  • Gene/Insert name
    24xMS2 repeats derived from Addgene plasmid # 31865
  • Insert Size (bp)
    1250

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Digest with NotI for transfection into trypanosomes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    prDNA spacer-LacOx120-20xMS2-Hyg was a gift from Calvin Tiengwe (Addgene plasmid # 210220 ; http://n2t.net/addgene:210220 ; RRID:Addgene_210220)

  • For your References section:

    Imaging of genomic loci in Trypanosoma brucei using an optimised LacO-LacI system. Budzak J, Goodwin I, Tiengwe C, Rudenko G. Mol Biochem Parasitol. 2023 Dec;256:111598. doi: 10.1016/j.molbiopara.2023.111598. Epub 2023 Nov 3. 10.1016/j.molbiopara.2023.111598 PubMed 37923299
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