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Addgene

MEIS1B-MSCV
(Plasmid #21022)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21022 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MEIS1B
  • Alt name
    murine ecotropic insertion site 1B
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1446
  • Entrez Gene
    Meis1 (a.k.a. C530044H18Rik, Evi8)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN-5
  • 3′ sequencing primer MSCV-rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Arthur Buchberg

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MEIS1B-MSCV was a gift from Corey Largman (Addgene plasmid # 21022 ; http://n2t.net/addgene:21022 ; RRID:Addgene_21022)

  • For your References section:

    HOXA9 forms triple complexes with PBX2 and MEIS1 in myeloid cells. Shen WF, Rozenfeld S, Kwong A, Koem ves LG, Lawrence HJ, Largman C. Mol Cell Biol. 1999 Apr . 19(4):3051-61. PubMed 10082572
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