prDNA spacer-LacOx50-20xMS2-Hyg
(Plasmid
#210219)
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PurposeIntegrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 210219 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-3
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2413
- Total vector size (bp) 8225
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert name50xLacO repeats derived from: PMID: 12864855.
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SpeciesSynthetic; E. coli
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Insert Size (bp)1887
Gene/Insert 2
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Gene/Insert name24xMS2 repeats derived from Addgene plasmid # 31865
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Insert Size (bp)1250
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Digest with NotI for transfection into trypanosomes.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
prDNA spacer-LacOx50-20xMS2-Hyg was a gift from Calvin Tiengwe (Addgene plasmid # 210219 ; http://n2t.net/addgene:210219 ; RRID:Addgene_210219) -
For your References section:
Imaging of genomic loci in Trypanosoma brucei using an optimised LacO-LacI system. Budzak J, Goodwin I, Tiengwe C, Rudenko G. Mol Biochem Parasitol. 2023 Dec;256:111598. doi: 10.1016/j.molbiopara.2023.111598. Epub 2023 Nov 3. 10.1016/j.molbiopara.2023.111598 PubMed 37923299
Map uploaded by the depositor.
