mTB3-Myc tag
(Plasmid
#210205)
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PurposeType 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210205 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemTB3
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Backbone manufacturerAddgene ID: 206144
- Backbone size w/o insert (bp) 8264
- Total vector size (bp) 8321
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Modifications to backboneInsertion of the Myc-tag at the N-terminus of mature protein III.
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Vector typeBacterial Expression
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Selectable markerslacZα
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMyc-tag
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SpeciesSynthetic
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Insert Size (bp)30
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mTB3-Myc tag was a gift from Brian Kay (Addgene plasmid # 210205 ; http://n2t.net/addgene:210205 ; RRID:Addgene_210205) -
For your References section:
Efficient Cloning of Inserts for Phage Display by Golden Gate Assembly. Grahn AK, Allen GL, Kay BK. Methods Mol Biol. 2023;2702:191-203. doi: 10.1007/978-1-0716-3381-6_9. 10.1007/978-1-0716-3381-6_9 PubMed 37679620