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Addgene

mTB3-FLAG tag
(Plasmid #210204)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210204 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mTB3
  • Backbone manufacturer
    Addgene ID: 206144
  • Backbone size w/o insert (bp) 8264
  • Total vector size (bp) 8273
  • Modifications to backbone
    Insertion of the FLAG-tag at the N-terminus of mature protein III.
  • Vector type
    Bacterial Expression
  • Selectable markers
    lacZα

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FLAG-tag
  • Species
    Synthetic
  • Insert Size (bp)
    24

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mTB3-FLAG tag was a gift from Brian Kay (Addgene plasmid # 210204 ; http://n2t.net/addgene:210204 ; RRID:Addgene_210204)

  • For your References section:

    Efficient Cloning of Inserts for Phage Display by Golden Gate Assembly. Grahn AK, Allen GL, Kay BK. Methods Mol Biol. 2023;2702:191-203. doi: 10.1007/978-1-0716-3381-6_9. 10.1007/978-1-0716-3381-6_9 PubMed 37679620
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