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Addgene

LentiV2-EF1α-nCas9/RT
(Plasmid #210188)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210188 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiCRISPR v2 (Plasmid #52961)
  • Backbone size w/o insert (bp) 14873
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nCas9/RT-P2A-Puro
  • Insert Size (bp)
    7065

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LentiV2-EF1α-nCas9/RT was a gift from Yin Shen (Addgene plasmid # 210188 ; http://n2t.net/addgene:210188 ; RRID:Addgene_210188)

  • For your References section:

    High-throughput PRIME-editing screens identify functional DNA variants in the human genome. Ren X, Yang H, Nierenberg JL, Sun Y, Chen J, Beaman C, Pham T, Nobuhara M, Takagi MA, Narayan V, Li Y, Ziv E, Shen Y. Mol Cell. 2023 Dec 21;83(24):4633-4645.e9. doi: 10.1016/j.molcel.2023.11.021. 10.1016/j.molcel.2023.11.021 PubMed 38134886
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