pRH3186
(Plasmid
#210181)
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PurposeProtein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W353G-GFP::tADH1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210181 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRH3022
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYIp ADE2-LEU2 pTDH3::lys1-W353G-GFP::tADH1
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SpeciesS. cerevisiae (budding yeast)
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MutationW353G
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Entrez GeneLYS1 (a.k.a. YIR034C)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRH3186 was a gift from Randy Hampton (Addgene plasmid # 210181 ; http://n2t.net/addgene:210181 ; RRID:Addgene_210181) -
For your References section:
Exploring the "misfolding problem" by systematic discovery and analysis of functional-but-degraded proteins. Flagg MP, Lam B, Lam DK, Le TM, Kao A, Slaiwa YI, Hampton RY. Mol Biol Cell. 2023 Dec 1;34(13):ar125. doi: 10.1091/mbc.E23-06-0248. Epub 2023 Sep 20. 10.1091/mbc.E23-06-0248 PubMed 37729018