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Addgene

pRH3047
(Plasmid #210157)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210157 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRH3022
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YIp ADE2-LEU2 pTDH3::lys1-W151H-GFP::tADH1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    W151H
  • Entrez Gene
    LYS1 (a.k.a. YIR034C)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRH3047 was a gift from Randy Hampton (Addgene plasmid # 210157 ; http://n2t.net/addgene:210157 ; RRID:Addgene_210157)

  • For your References section:

    Exploring the "misfolding problem" by systematic discovery and analysis of functional-but-degraded proteins. Flagg MP, Lam B, Lam DK, Le TM, Kao A, Slaiwa YI, Hampton RY. Mol Biol Cell. 2023 Dec 1;34(13):ar125. doi: 10.1091/mbc.E23-06-0248. Epub 2023 Sep 20. 10.1091/mbc.E23-06-0248 PubMed 37729018
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