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Addgene

MEIS1A-MIY
(Plasmid #21013)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21013 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV-IRES-YFP
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MEIS1A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Entrez Gene
    Meis1 (a.k.a. C530044H18Rik, Evi8)
  • Tags / Fusion Proteins
    • IRES (C terminal on backbone)
    • YFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN-5
  • 3′ sequencing primer MSCV-rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Meis1A from Arthur Buchberg, YFP-IRES from Keith Humphries
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MEIS1A-MIY was a gift from Corey Largman (Addgene plasmid # 21013 ; http://n2t.net/addgene:21013 ; RRID:Addgene_21013)

  • For your References section:

    HOXB6 overexpression in murine bone marrow immortalizes a myelomonocytic precursor in vitro and causes hematopoietic stem cell expansion and acute myeloid leukemia in vivo. Fischbach NA, Rozenfeld S, Shen W, Fong S, Chrobak D, Ginzinger D, Kogan SC, Radhakrishnan A, Le Beau MM, Largman C, Lawrence HJ. Blood. 2005 Feb 15. 105(4):1456-66. 10.1182/blood-2004-04-1583 PubMed 15522959
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