PRRX2-pBLSK
(Plasmid #21012)

• Depositing Lab: Corey Largman
• Publication: Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Backbone

• Vector backbone: pBluescript SK
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PRRX2
• Species: M. musculus (mouse)
• Insert Size (bp): 1200
• Mutation: Missing first three amino acids at N-terminus
• Entrez Gene: Prrx2 (a.k.a. S8, Prx2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: BglII/BamHI (blunt) (destroyed during cloning)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Fritz Meijlink
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry