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Addgene

PRRX2-pET
(Plasmid #21011)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21011 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRRX2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1220
  • Mutation
    Missing first 3 N-terminal amino acids
  • Entrez Gene
    Prrx2 (a.k.a. S8, Prx2)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • T7-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-terminal
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Fritz Meijlink

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PRRX2-pET was a gift from Corey Largman (Addgene plasmid # 21011 ; http://n2t.net/addgene:21011 ; RRID:Addgene_21011)

  • For your References section:

    Modulation of the human homeobox genes PRX-2 and HOXB13 in scarless fetal wounds. Stelnicki EJ, Arbeit J, Cass DL, Saner C, Harrison M, Largman C. J Invest Dermatol 1998 Jul;111(1):57-63. 10.1046/j.1523-1747.1998.00238.x PubMed 9665387
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