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Addgene

PRRX2-MSCV
(Plasmid #21010)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21010 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PRRX2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Entrez Gene
    Prrx2 (a.k.a. S8, Prx2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BglII/BamHI (blunt) (destroyed during cloning)
  • 5′ sequencing primer MSCV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PRRX2-MSCV was a gift from Corey Largman (Addgene plasmid # 21010 ; http://n2t.net/addgene:21010 ; RRID:Addgene_21010)

  • For your References section:

    Modulation of the human homeobox genes PRX-2 and HOXB13 in scarless fetal wounds. Stelnicki EJ, Arbeit J, Cass DL, Saner C, Harrison M, Largman C. J Invest Dermatol 1998 Jul;111(1):57-63. 10.1046/j.1523-1747.1998.00238.x PubMed 9665387
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