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pPB9
(Plasmid #210036)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210036 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    recommend using LB with 100 µg/mL carbenicillin
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Cas9
  • Species
    S. pyogenes
  • Insert Size (bp)
    4101
  • Promoter TRE3G
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on backbone)
    • nucleoplasmin NLS (C terminal on backbone)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    puro-T2A-eGFP
  • Species
    Synthetic
  • Insert Size (bp)
    1365
  • Promoter EF-1α

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB9 was a gift from Michael Talkowski (Addgene plasmid # 210036 ; http://n2t.net/addgene:210036 ; RRID:Addgene_210036)

  • For your References section:

    Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR libraries. Nuttle X, Burt ND, Currall B, Moyses-Oliveira M, Mohajeri K, Bhavsar R, Lucente D, Yadav R, Tai DJC, Gusella JF, Talkowski ME. Cell Rep Methods. 2023 Dec 5:100672. doi: 10.1016/j.crmeth.2023.100672. 10.1016/j.crmeth.2023.100672 PubMed 38091988
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