HOXD4-WV-MSCV
(Plasmid
#21002)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21002 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMSCV
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 6500
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Vector typeMammalian Expression, Retroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHOXD4
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SpeciesM. musculus (mouse)
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Insert Size (bp)1000
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MutationW->V PBX1 interaction mutant
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Entrez GeneHoxd4 (a.k.a. 6030436D05Rik, Hox-4.2, Hox-5.1)
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Tags
/ Fusion Proteins
- His (N terminal on insert)
- T7-tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (blunted) (destroyed during cloning)
- 3′ cloning site XbaI (blunted) (destroyed during cloning)
- 5′ sequencing primer MSCV (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMark Featherstone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mutation prevents interaction with PBX proteins
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HOXD4-WV-MSCV was a gift from Corey Largman (Addgene plasmid # 21002 ; http://n2t.net/addgene:21002 ; RRID:Addgene_21002)