pLPC52
(Plasmid
#209991)
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PurposeContains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmids
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209991 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLP1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert name5' Connector (5C2SN)
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Original Marburg Collection name: pMC0_1_08_5C2SN
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLPC52 was a gift from Georg Fritz & Thorsten Mascher (Addgene plasmid # 209991 ; http://n2t.net/addgene:209991 ; RRID:Addgene_209991) -
For your References section:
The Marburg Collection: A Golden Gate DNA Assembly Framework for Synthetic Biology Applications in Vibrio natriegens. Stukenberg D, Hensel T, Hoff J, Daniel B, Inckemann R, Tedeschi JN, Nousch F, Fritz G. ACS Synth Biol. 2021 Aug 20;10(8):1904-1919. doi: 10.1021/acssynbio.1c00126. Epub 2021 Jul 13. 10.1021/acssynbio.1c00126 PubMed 34255476