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Addgene

pLPE1
(Plasmid #209981)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209981 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Vector type
    Bacterial Expression
  • Selectable markers
    mScarlet-I

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    E. coli shuttle part (pMB1_ampR_B0017)
  • Species
    E. coli

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

constructed by Golden Gate cloning mediated ligation of multiple PCR product, not from an entry vector

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPE1 was a gift from Thorsten Mascher (Addgene plasmid # 209981 ; http://n2t.net/addgene:209981 ; RRID:Addgene_209981)

  • For your References section:

    Development of a Golden Gate Assembly-Based Genetic Toolbox for Lactiplantibacillus plantarum and Its Application for Engineering Monoterpenoid Biosynthesis. Li X, Schönberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, Mascher T. ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 10.1021/acssynbio.4c00075. Epub 2024 Sep 10. 10.1021/acssynbio.4c00075 PubMed 39254046
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